This study aimed to develop an active edible film based on carboxymethyl chitosan (CMCS) and pullulan (Pul) incorporated with galangal essential oil (GEO) by the casting method. https://www.selleckchem.com/products/darapladib-sb-480848.html And their physical properties, structural and preservation effect on mangoes were characterized. The CMCS/Pul ratio was determined to be 2.52.5 after the optimization of physical properties, mechanical properties and barrier properties of the blend film. The results of FT-IR and XRD showed that hydroxyl groups of Pul interacted with the carboxyl groups of CMCS and the blend films had good compatibility. Good thermal stability of CMCS/Pul-GEO films was further proven by TGA curves. The CMCS/Pul-8 %GEO film showed effective preservations on mango fruits during 15 days of storage at 25 ± 1 °C, based on the characterization by fruits weight loss, firmness, titratable acidity, soluble solids. Consequently, CMCS/Pul-GEO blend films may be a promising eco-friendly packaging material for the industrial application of fruit preservation.In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body. Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate. It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration. The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC). The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC. The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment. Results indicated that all prepared dural substitutes were tightly bound between layers without excessively large cavities. The porous layer showed appropriate pore size (90〜200 μm) with high porous connectivity. The optimized bilayer dural substitutes showed suitable swelling rate and mechanical behavior. Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties. Also, it was observed that it did not deform in the peritoneal environment of mice, and tissue inflammation was mild.Chitosans with different average degrees of acetylation and weight molecular weight were analyzed by time-domain NMR relaxometry using the recently proposed pulse sequence named Rhim and Kessemeier - Radiofrequency Optimized Solid-Echo (RK-ROSE) to acquire 1H NMR signal of solid-state materials. The NMR signal decay was composed of faster (tenths of μs) and longer components, where the mobile-part fraction exhibited an effective relaxation transverse time assigned to methyl hydrogens from N-acetyl-d-glucosamine (GlcNAc) units. The higher intrinsic mobility of methyl groups was confirmed via DIPSHIFT experiments by probing the 1H-13C dipolar interaction. RK-ROSE data were modeled by using Partial Least Square (PLS) multivariate regression, which showed a high coefficient of determination (R2 &gt; 0.93) between RK-ROSE signal profile and average degrees of acetylation and crystallinity index, thus indicating that time-domain NMR consists in a promising tool for structural and morphological characterization of chitosan.Chitosan-modified zinc hydroxystannate (ZHS-CS) was synthesized using the cations of the biomaterial chitosan (CS) and ion replacement strategy. A ZHS-CS and reduced graphene oxide (rGO) hybrid flame retardant (ZHS-CS/rGO) was synthesized for use in flexible poly (vinyl chloride) (PVC). Scanning electron microscopy images indicated that ZHS-CS and rGO were evenly dispersed in ZHS-CS/rGO without agglomeration. Fourier transform infrared spectroscopy results showed that rGO was fully reduced. The flame-retardant and mechanical properties of PVC composites were investigated using the limiting oxygen index (LOI), a cone calorimeter, and mechanical equipment. By replacing one-fifth of the zinc ions in ZHS by chitosan cations to obtain Sn-4Zn-1CS/rGO, the ZHS-CS/rGO was found to improve PVC composite performance. The total heat release and total smoke release of PVC/Sn-4Zn-1CS/rGO were reduced by 24.2 and 40.0 %, respectively, from those of pure PVC.To meet the demands of various therapeutic tasks, injectable hydrogels with tunable mechanical properties and degradability are highly desired. Herein, we developed an injectable chitin hydrogel system with well-manipulated mechanical properties and degradability through dynamic acylhydrazone crosslinking catalyzed by 4-amino-DL-phenylalanine (Phe-NH2). The mechanical properties and degradability of the hydrogels could be easily adjusted by varying the solid content, while their gelation time could be maintained at a constant level (?130 s) by altering Phe-NH2 content, thereby ensuring the good injectability of hydrogels. Moreover, the chitin hydrogels showed excellent self-healing capacity with a healing efficiency up to 95 %. Owing to their superior biocompatibility and biodegradability, the chitin hydrogels could support the proliferation and multi-potent differentiations of rat bone marrow-derived stem cells, serving as a beneficial 3D scaffold for stem cell encapsulation and delivery. This work provides a promising injectable delivery vehicle of therapeutic drugs or cells for tissue regenerative medicine.Aloe polysaccharides (APs) are well-known plant polysaccharides, but little is known about their digestion and fermentation characteristics in vitro. In this study, the molecular weight of APs had no significant changes after gastric and intestinal digestion. During the fecal fermentation, the content of volatiles and pH value decreased continuously, while the short-chain fatty acids (SCFAs) concentration increased significantly. Additionally, the abundance of the microbiota associated with the metabolism of SCFAs was increased, including Prevotella, Catenibacterium, Lachnospiraceae, and Coprococcus, while the harmful microbiota was decreased, like Escherichia-Shigella, and Veillonella. Moreover, bioinformatics analysis indicated that APs boosted fructose and mannose metabolism, and the gene expressions of enzymes, containing mannose-6-phosphate isomerase [EC5.3.1.8]. Structural equation modeling also highlighted that SCFAs-producing microbiota were primary degraders of APs, suggesting APs may facilitate the manufacture of functional foods with the purpose of maintaining intestinal health.